期刊
VIRUSES-BASEL
卷 5, 期 10, 页码 2601-2613出版社
MDPI
DOI: 10.3390/v5102601
关键词
PEDV; S gene; heterogeneity; genetic variation; variants
类别
资金
- National Natural Science Foundation of China [31172350, 30901081]
- Central Public-Interest Scientific Institution Basal Research Fund [0302013002]
- Natural Science Foundation for Distinguished Young Scholars of Heilongjiang province [JC201118]
- Agricultural Scientific and Technological Transformative Project [2011GB23260003]
- Higher school science and technology innovation team project of Heilongjiang province [2011TD001]
- Technology Research and Development Program of Harbin [2009AA6AN029]
Since late 2010, porcine epidemic diarrhea virus (PEDV) has rapidly disseminated all over the China and caused considerable morbidity and high mortality (up to 100%) in neonatal piglets. 79.66% (141 of 177) pig farms in 29 provinces (excluding Tibet and Hainan, China) and 72.27% (417 of 577) samples were positive for PEDV confirmed by reverse transcription-polymerase chain reaction (RT-PCR). The full-length S genes of representative field strains were sequenced. 33 field strains share 93.5%-99.9% homologies with each other at the nucleotide sequence level and 92.3%-99.8% homologies with each other at the amino acids sequence level. Most field strains have nucleotide deletion and insertion regions, and show lower homologies (93.5%-94.2%) with Chinese classical strain CH/S, however higher homologies (97.1%-99.3%) with recent strain CHGD-1. The phylogenetic analysis showed there are classical strains and variants prevailing in pig herd in China. PEDV has a high detection rate in pig herds in China. Sequence analysis indicated the S genes of recent field strains have heterogeneity and the variants are predominant.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据