期刊
VIRUSES-BASEL
卷 5, 期 2, 页码 663-677出版社
MDPI AG
DOI: 10.3390/v5020663
关键词
PRRSV; non-structural proteins; epitopes; interferon gamma; IL-10
类别
资金
- European Union [245141]
- Spanish Ministry of Economy and Competitiveness [CSD-0007]
- CONACYT [32796]
- Department of Economy and Knowledge of Generalitat de Catalunya [PIV2010-00037]
- Spanish Ministry of Education
- Consolider-Ingenio PORCIVIR
This work describes peptides from non-structural proteins (nsp) of porcine reproductive and respiratory syndrome virus (PRRSV) predicted as potential T cell epitopes by bioinfornatics and tested for their ability to induce IFN-gamma and IL-10 responses. Pigs immunized with either genotype 1 or genotype 2 PRRSV attenuated vaccines (n=5/group) and unvaccinated pigs (n=4) were used to test the peptides. Swine leukocyte antigen haplotype of each pig was also determined. Pigs were initially screened for IFN-gamma responses (ELISPOT) and three peptides were identified; two of them in non-conserved segments of nsp2 and nsp5 and the other in a conserved region of nsp5 peptide. Then, peptides were screened for IL-10 inducing properties. Six peptides were found to induce IL-10 release in PBMC and some of them were also able to inhibit IFN-gamma responses on PHA-stimulated cells. Interestingly, the IFN-gamma low responder pigs against PRRSV were mostly homozygous for their SLA haplotypes. In conclusion, these results indicate that nsp of PRRSV contain T-cell epitopes inducing IFN-gamma responses as well as IL-10 inducing segments with inhibitory capabilities.
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