期刊
FOOD AND ENVIRONMENTAL VIROLOGY
卷 5, 期 3, 页码 135-143出版社
SPRINGER
DOI: 10.1007/s12560-013-9112-6
关键词
Foodborne viruses; Heat inactivation; Food matrix; Murine norovirus; MS2 bacteriophage
资金
- German Ministry of Economics and Technology (via AiF)
- FEI (Research Association of the German Food Industry, Bonn)
- BVDF (Federal Association of the German Meat Industry)
- Project AiF [16479 BR]
Until now, little is known about the influence of food additives on heat inactivation of noroviruses. Only a few studies have shown a protective or inhibiting effect on virus infectivity caused by the food matrix. Therefore, the aim of this study was to examine the influence of sodium chloride, sucrose and milk on heat stability of the surrogates murine norovirus (MNV) and MS2 phage at 60 A degrees C for 1-5 min in PBS for MNV and for 5-120 min in suspension medium buffer for MS2 phage. Different concentrations of sodium chloride (5, 10 %) and sucrose (5, 50 %) were added to the respective buffers. In addition, commercially available milk with different fat concentrations (0.3, 1.5, 3.5 %) was investigated in this study. In general, a linear titre reduction for MNV and MS2 phage could be observed, except for the heat treatment of MNV in PBS with 50 % sucrose. A protective effect of PBS with 50 % sucrose and of the matrix milk on MNV could be concluded. All other tested conditions did not show any influence on virus inactivation. However, MS2 phage did show a higher heat resistance throughout the experiments compared to MNV. In future investigations, it should be tested, whether the achieved data may be considered in risk assessments of heat-treated food products with high concentrations of sugar. Furthermore, it should be clarified, whether these results can also be referred to complex food matrices.
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