期刊
ZYGOTE
卷 23, 期 4, 页码 475-484出版社
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S0967199414000070
关键词
Alginate; Follicle culture; Oocyte; Pre-antral follicle; Steroid secretion
资金
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) (Biotecnologia-Rede Nordeste em Biotecnologia (REN-ORBIO)) [554812/2006-1]
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) Brazil
- EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT [U54HD076188, P50HD076188] Funding Source: NIH RePORTER
The aim of this study was to evaluate the influence of two-dimensional (2D) and three-dimensional (3D) alginate culture systems on in vitro development of pre-antral caprine follicles. In addition, the influence of the reproductive age of the ovary donor on the in vitro culture success was investigated. Pre-antral follicles from pre-pubertal or adult goats were isolated and cultured directly on a plastic surface (2D) or encapsulated in an alginate-based matrix (3D). After 18 days, the oocytes underwent in vitro maturation (IVM) and in vitro fertilization (IVF) to produce embryos. The 3D system showed higher rates of follicle survival, lower rates of oocyte extrusion, and a greater number of recovered oocytes for IVM and IVF (P < 0.05). Only pre-antral follicles from adult animals produced MII oocytes and embryos. The estradiol concentrations increased from day 2 to day 12 of culture in all groups tested (P < 0.05). Conversely, progesterone concentrations were lower in 3D-cultured follicles than in 2D-cultured follicles, with differences on days 2 and 6 of culture (P < 0.05). We provide compelling evidence that a 2D or 3D alginate in vitro culture system offers a promising approach to achieving full in vitro development of caprine pre-antral follicles to produce mature oocytes that are capable of fertilization and viable embryos.
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