期刊
YONSEI MEDICAL JOURNAL
卷 52, 期 6, 页码 999-1007出版社
YONSEI UNIV COLL MEDICINE
DOI: 10.3349/ymj.2011.52.6.999
关键词
Lipoaspirates; adipose tissue; mesenchymal stem cell; proliferation; differentiation
资金
- Lifeliver. Co., Ltd.
Purpose: This study examined a rapid isolation method decreasing the time and cost of the clinical application of adipose tissue-derived stem cells (ASCs). Materials and Methods: Aliquots (10 g) of the lipoaspirates were stored at 4 degrees C without supplying oxygen or nutrients. At the indicated time points, the yield of mononuclear cells was evaluated and the stem cell population was counted by colony forming unit-fibroblast assays. Cell surface markers, stem cell-related transcription factors, and differentiation potentials of ASCs were analyzed. Results: When the lipoaspirates were stored at 4 degrees C, the total yield of mononuclear cells decreased, but the stem cell population was enriched. These ASCs expressed CD44, CD73, CD90, CD105, and HLA-ABC but not CD14, CD31, CD34, CD45, CD117, CD133, and HLA-DR. The number of ASCs increased 1 x 10(14) fold for 120 days. ASCs differentiated into osteoblasts, adipocytes, muscle cells, or neuronal cells. Conclusion: ASCs isolated from lipoaspirates and stored for 24 hours at 4 degrees C have similar properties to ASCs isolated from fresh lipoaspirates. Our results suggest that ASCs can be isolated with high frequency by optimal storage at 4 degrees C for 24 hours, and those ASCs are highly proliferative and multipotent, similar to ASCs isolated from fresh lipoaspirates. These ASCs can be useful for clinical application because they are time- and cost-efficient, and these cells maintain their sternness for a long time, like ASCs isolated from fresh lipoaspirates.
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