期刊
YONSEI MEDICAL JOURNAL
卷 50, 期 3, 页码 399-406出版社
YONSEI UNIV COLL MEDICINE
DOI: 10.3349/ymj.2009.50.3.399
关键词
Heak shock protein; cervical cancer; prognostic marker
Purpose: Cervical cancer caused by the human papilloma virus (HPV) continues to be the cause of yearly death among women. However, it is a curable disease when diagnosed at an early stage. Recently, several researches have reported that heat shock protein (HSP) 60, a chaperone protein of molecular weight of 60 kDa, is involved in carcinogenesis and apoptosis. In order to evaluate the prognostic significance of HSP60 in cervical cancer, we examined differences in the HSP60 expression between cervical cancer and normal tissues in women. Materials and Methods: Tissue samples were collected from 20 cervical cancer patients and 20 normal controls. HSP60 expression of cervical cancer and normal tissues were verified by the 2D gel proteomics, semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) and Western blot analyses. Results: In 2D proteomic: analysis, an increase of HSP60 expression was detected in cervical cancer tissues and confirmed by Western blot analysis (p < 0.05). However, messenger RNA (mRNA) levels of HSP60 did not display any significant differences between cervical cancer and normal tissues. Conclusion: These results suggest that HSP60 may be involved in the development of cervical cancer and have profound biological and prognostic significance.
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