期刊
YEAST
卷 30, 期 5, 页码 165-177出版社
WILEY
DOI: 10.1002/yea.2954
关键词
non-Saccharomyces yeast; lactose metabolism; glucose signalling; MAPK signalling; heterologous gene expression
资金
- Deutsche Forschungsgemeinschaft [SFB 944]
- MICINN, Spain [BFU2010-19628-C02-01]
The milk yeast Kluyveromyces lactis has a life cycle similar to that of Saccharomyces cerevisiae and can be employed as a model eukaryote using classical genetics, such as the combination of desired traits, by crossing and tetrad analysis. Likewise, a growing set of vectors, marker cassettes and tags for fluorescence microscopy are available for manipulation by genetic engineering and investigating its basic cell biology. We here summarize these applications, as well as the current knowledge regarding its central metabolism, glucose and extracellular stress signalling pathways. A short overview on the biotechnological potential of K. lactis concludes this review. Copyright (c) 2013 John Wiley & Sons, Ltd.
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