期刊
YEAST
卷 27, 期 12, 页码 999-1003出版社
WILEY
DOI: 10.1002/yea.1808
关键词
Saccharomyces cerevisiae; zymolyase; ether; tetrad; selection; polyploid; offspring; haploid
Here we describe a new procedure for ascospore isolation from cultures containing a majority of unsporulated vegetative cells of Saccharomyces cerevisiae. The EZ ascospore isolation procedure relies on the combination of two conventional protocols, diethyl ether treatment and modified zymolyase treatment, allowing a significant increase in the efficiency of ascospore isolation and consequently enabling a large number of meiotic offspring to be efficiently obtained and screened, thus improving the efficacy of genetic research and the genetic selection of S. cerevisiae strains. Copyright (C) 2010 John Wiley & Sons, Ltd.
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