期刊
XENOBIOTICA
卷 42, 期 11, 页码 1120-1127出版社
TAYLOR & FRANCIS LTD
DOI: 10.3109/00498254.2012.689887
关键词
Osthole; metabolites; hydroxylation; glucuronidation; hydrogenation
资金
- National Natural Science Foundation of China [30701088, 81073013]
- Education Department of Liaoning Province [LS2010059]
- Personnel Department of Liaoning province
- Program for Liaoning Excellent Talents in the University [LJQ2011095]
1. Osthole (Ost), one of the major components of Cnidium monnieri (L.) Cusson, is had the structure of an isopentenoxy-coumarin with a range of pharmacological activities. In the present study, the metabolism of Ost in male Sprague-Dawley rats was investigated by identifying Ost metabolites excreted in rat urine. 2. Following an oral dose of 40 mg/kg Ost, 10 phase I and 3 phase II metabolites were isolated from the urine of rats, and their structures identified on the basis of a range of spectroscopic data, including 2D-NMR techniques. These metabolites were fully characterized as 5'-hydroxyl-osthole (M-1), osthenol (M-2), 4'-hydroxyl-osthole (M-3), 3, 5'-dihydroxyl-osthole (M-4), 5'-hydroxyl-osthenol (M-5), 4'-hydroxyl-2', 3'-dihydro-osthenol (M-6), 4'-hydroxyl-osthenol (M-7), 3, 4'-dihydroxyl-osthole (M-8), 2', 3'-dihydroxyl-osthole (M-9), 5'-hydroxyl-2', 3'-dihydroosthole (M-10), osthenol-7-O-beta-D-glucuronide (M-11), osthole-4'-O-beta-D-glucuronide (M-12) and osthole-5'-O-beta-D-glycuronate (M-13). This is the first identification of M-1, M-3 to M-13 in vivo. 3. On the basis of the metabolites profile, a possible metabolic pathway for Ost metabolism in rats has been proposed. This is the first systematic study on the phases I and II metabolites of 8-isopentenoxy-coumarin derivative.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据