期刊
WOUND REPAIR AND REGENERATION
卷 18, 期 5, 页码 506-513出版社
WILEY-BLACKWELL
DOI: 10.1111/j.1524-475X.2010.00616.x
关键词
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资金
- National Natural Science Foundation of China [30672174, 30973116]
- 863 project in China [2006AA02A121]
- State Key Laboratory of Trauma, Burns and Combined Injury [SK LZZ200808, SKLZZ200820]
- CQSTC [2005BB5044]
- Military Medical Program [06H031, 06-Z-054]
- Southwest Hospital and Third Military Medical University [SWH2005A010]
This study aimed to determine whether mesenchymal stem cells (MSCs) derived from umbilical cord blood (UCB) would promote cutaneous wound healing. MSCs from human UCB were isolated and identified. The characteristics of the isolated MSCs' growth and proliferation were assayed in vitro. The MSCs labeled with 5-bromodeoxyuridine (BrdU) were applied on fresh cutaneous mice wounds. The healing rates were surveyed. The distribution and the differentiation into keratinocytes of the labeled MSCs in the wound tissue were checked by immunohistochemistry staining. The isolated MSCs could grow and proliferate well in vitro. The isolated MSCs from UCB could be labeled by 5-bromodeoxyuridine successfully. The MSCs derived from UCB could enhance the healing of mice skin defect wounds, and it was found that the implanted MSCs could differentiate into keratinocyte in the wound tissue. It was demonstrated that MSCs from UCB can be isolated and proliferated successfully. The local administration of MSCs derived from UCB improves skin defect wound healing in mice.
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