期刊
WORLD JOURNAL OF PEDIATRICS
卷 6, 期 2, 页码 132-140出版社
ZHEJIANG UNIV SCH MEDICINE
DOI: 10.1007/s12519-010-0019-0
关键词
bacillus Calmette-Guerin; cord blood; dendritic cells; newborns; T cells; tolerance
类别
资金
- Council of the Hong Kong Special Administrative Region, China (RCG) [HKU-7260/01M]
- Department of Pediatrics and Adolescent Medicine, The University of Hong Kong
Background: As one of the first infectious challenges of life, the impact of neonatal Mycobacterium bovis bacillus Calmette-Guerin (BCG) vaccination on the polarization of neonatal T helper subset has not been well defined. Methods: We investigated the effect of BCG-treated cord blood (CB) dendritic cells (DCs) on naive CD4+ T cells polarization compared with that of adult blood DCs. Results: BCG-treated CB DCs had significantly lower expression of CD83 and a higher ratio of CD47/Fas than BCG-treated adult blood DCs. BCG induced significantly lower IL-12 but relatively higher IL-10 production from CB DCs than adult blood DCs. Moreover, in comparison with BCG-treated adult blood DCs, BCG-treated CB DCs induced higher IL-10 production and cytotoxic T-lymphocyte antigen 4 (CTLA-4) expression, and lower interferon-gamma (IFN-gamma) production from naive CD4+ T cells. On the other hand, lipopolysaccharide-treated CB DCs had similar capacity as prime naive CD4+ T cells did to produce higher IFN-gamma, lower IL-10 production, and CTLA-4 expression compared with their adult counterparts. Conclusion: These results suggested that BCG-treated CB DCs might be semi-mature DCs which polarize naive T cells into a tolerogenic T cell phenotype in newborns.
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