期刊
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
卷 30, 期 11, 页码 2967-2973出版社
SPRINGER
DOI: 10.1007/s11274-014-1724-y
关键词
5-Enopyruvylshikimate-3-phosphate synthase; Sinorhizobium fredii; Glyphosate-tolerant; Class II EPSP synthase
资金
- Shanghai Municipal Committee of Agriculture [2011-1-8]
- International Scientific and Technological Cooperation [2010DFA62320, 11230705900]
- Youth Fund of Shanghai Academy of Agricultural Sciences [2012-16, 2013-16]
- National Natural Science Foundation [31071486]
- Shanghai Minhang Science and Technology Committee [2012MH059]
5-Enopyruvylshikimate-3-phosphate synthase (EPSP synthase) is an important enzyme in the shikimate pathway mediating the biosynthesis of aromatic compounds in plants and microorganisms. A novel class II EPSP synthase AroA (S. fredii) from Sinorhizobium fredii NGR234 was overexpressed in Escherichia coli BL21. It was purified to homogeneity and its catalytic properties were studied. The enzyme exhibited optimum catalytic activity at pH 8.0 and 50 A degrees C. It was stable below 40 A degrees C, and over a broad range of pH 5.0-9.0. The EPSP synthase was increasingly activated by 100 mM of the chlorides of NH4 (+), K+, Na+ and Li+. Kinetic analysis of AroA (S. fredii) suggested that the enzyme exhibited a high glyphosate tolerance and high level of affinity for phosphoenolpyruvate, which indicates the enzyme with a high potential for structural and functional studies and its potential usage for the generation of transgenic crops resistant to the herbicide.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据