期刊
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
卷 30, 期 1, 页码 175-180出版社
SPRINGER
DOI: 10.1007/s11274-013-1432-z
关键词
Catharanthus roseus; Suspension-cultured cells; Vindoline; Vinblastine; Artemisinic acid; Elicitation
资金
- National Natural Science Foundation of China [81073004, 81102771]
- Guangdong Natural Science Foundation [S2011040003028]
Elicitation is an important strategy to improve production of secondary metabolites in vitro. Artemisinic acid was studied as a novel elicitor to enhance the yield of terpenoid indole alkaloids in the present paper. Our results demonstrated that the concentrations of vindoline and vinblastine were increased by sixfold and twofold, respectively, compared to those of the control group after treatment with artemisinic acid. To elucidate the underlying mechanism, we investigated the gene expression of four enzymes involved in the biosynthetic pathway of vinblastine in the suspension-cultured cells of Catharanthu sroseus. RT-PCR experiment showed that artemisinic acid was able to up-regulate the transcriptions of tryptophan decarboxylase, geraniol 10-hydroxylase, tabersonine 16-hydroxylase and deacetoxyvindoline 4-hydroxylase.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据