期刊
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
卷 29, 期 11, 页码 2167-2172出版社
SPRINGER
DOI: 10.1007/s11274-013-1382-5
关键词
Arginase; Whole-cell; Biotransformation; L-Arginine; L-Ornithine
资金
- Jiangsu Planned Projects for Postdoctoral Research Funds [1101005C]
- Special Research of Environmental Nonprofit Industry [201009023]
- Open Fund of State Key Laboratory of Pharmaceutical Biotechnology of Nanjing University, China
A recombinant arginase was generated for a whole-cell biotransformation system to convert l-arginine to l-ornithine in Escherichia coli. The gene ARG1 coding arginase from Bos taurus liver was synthesized and expressed in E. coli BL21 (DE3) via pETDuet-1. The recombinant arginase was used to catalyze l-arginine to l-ornithine and urea. The reaction was optimal at pH 9.5 and 37 A degrees C. Manganese (10(-5) M) and Emulsifier OP-10 [0.033 % (v/v)] could promote arginase activity. In a scale up study, l-arginine conversion rate reached 98 % with a final concentration of 111.52 g l-ornithine/l.
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