期刊
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
卷 28, 期 2, 页码 549-557出版社
SPRINGER
DOI: 10.1007/s11274-011-0846-8
关键词
Lysobacter enzymogenes; chiA; Suicide vector; Gene mutagenesis
资金
- Special Fund for Agro-scientific Research in the Public Interest [200903052, 201003004]
- Natural Science Foundation of Jiangsu Province [BK2010445]
- Ministry of Education of China [20100097120013]
- National High Technology Research and Development Program of China [2006AA10A211]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
Here, three different suicide vectors were evaluated for the possibility of performing gene mutagenesis in strain OH11 using the chiA gene (accession number: DQ888611) as a new reporter. Suicide vector pEX18GM was selected, and it was successfully applied for disruption and in-frame deletions in the chiA gene in strain OH11, which was confirmed by PCR amplification and Southern hybridization. The chiA-deletion mutant OH11-3 did not have the ability to produce chitinase on chitine selection medium. Interestingly, the chiA-deletion mutants displayed wild-type antimicrobial activity against Saccharomyces cerevisiae, Magnaporthe grisea, Phytophthora capsici, Rhizoctonia solani, Sclerotinia sclerotiorum and Pythium ultimum. Our data suggest that chitinase might not be a unique lytic enzyme in controlling S. cerevisiae, M. grisea, P. capsici, and P. ultimum. R. solani, S. sclerotiorum. Also, suicide vector pEX18GM might be explored as a potential tool for gene deletions in L. enzymogenes, which will facilitate the molecular study of mechanisms of biological control in L. enzymogenes.
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