期刊
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
卷 26, 期 5, 页码 817-823出版社
SPRINGER
DOI: 10.1007/s11274-009-0238-5
关键词
Serratia marcescens; Nitrile hydratase; 2-Cyanopyrazine; Pyrazinamide
资金
- Major Basic Research Development Program of China [2009CB724704]
- Zhejiang Province Natural Sciences Foundation of China [Y306205]
- Research Program of Science and Technology Department of Zhejiang Province [2007C33003]
Pyrazinamide has received considerable attention for its effective antibacterial action in the reappearance of tuberculosis and for its broad application in the chemical industry. In this study, a 2-cyanopyrazine-degrading bacterial strain, numbered ZJB-09104, was newly isolated and identified as Serratia marcescens, based on its physiological and biological tests, ATB system analysis, and 16S rDNA sequence analysis. The strain exhibits only nitrile hydratase (NHase) activity and this NHase belongs to the cobalt NHase family of enzymes. Thermostability tests suggested that the NHase is thermophilic with an optimum temperature of 50A degrees C. The NHase was effective in converting nitriles to the corresponding amides under the conditions of temperature 50A degrees C and time course 7 h, respectively.
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