期刊
WORLD JOURNAL OF GASTROENTEROLOGY
卷 19, 期 18, 页码 2818-2825出版社
BAISHIDENG PUBL GRP CO LTD
DOI: 10.3748/wjg.v19.i18.2818
关键词
Acute-on-chronic hepatitis B liver failure; Model for end-stage liver disease; CD163; Soluble CD163; Real-time transcription-polymerase chain reaction
资金
- Chinese Ministry of Science and Technology [2012ZX10002007, 2013ZX10002001]
- National Natural Science Foundation of China [81171579, 81201287]
- Natural Science Foundation of Shandong Province [ZR2010HM070, ZR2010HQ040]
AIM: To assess CD163 expression in plasma and peripheral blood and analyze its association with disease in acute-on-chronic hepatitis B liver failure (ACHBLF) patients. METHODS: A retrospective study was conducted from January 1, 2011 to January 1, 2012. Forty patients with ACHBLF (mean age 44.48 +/- 12.28 years, range 18-69 years), 40 patients with chronic hepatitis B (CHB) (mean age 39.45 +/- 12.22 years, range 21-57 years) and 20 age-and sex-matched healthy controls (mean age 38.35 +/- 11.97 years, range 28-60 years) were included in this study. Flow cytometry was used to analyze the frequency of CD163+ peripheral blood mononuclear cells (PBMCs) and surface protein expression of CD163. Real-time transcription-polymerase chain re-action was performed to assess relative CD163 mRNA levels in PBMCs. Plasma soluble CD163 (sCD163) levels were measured by enzyme-linked immunosorbent assay. Clinical variables were also recorded. Comparisons between groups were analyzed by Kruskal-Wallis H test and Mann-Whitney U test. Statistical analyses were performed using SPSS 15.0 software and a P value < 0.05 was considered statistically significant. RESULTS: Flow cytometry showed that the population of CD163+ PBMCs was significantly greater in ACHBLF patients than in CHB patients and healthy controls (47.9645% +/- 17.1542%, 32.0975% +/- 11.0215% vs 17.9460% +/- 6.3618%, P < 0.0001). However, there were no significant differences in mean fluorescence intensity of CD163+ PBMCs within the three groups (27.4975 +/- 11.3731, 25.8140 +/- 10.0649 vs 20.5050 +/- 6.2437, P = 0.0514). CD163 mRNA expression in ACHBLF patients was significantly increased compared with CHB patients and healthy controls (1.41 x 10(-2) +/- 2.18 x 10(-2), 5.10 x 10(-3) +/- 3.61 x 10(-3) vs 37.0 x 10(-4) +/- 3.55 x 10(-4), P = 0.02). Plasma sCD163 levels in patients with ACHBLF were significantly increased compared with CHB patients and healthy controls (4706.2175 +/- 1681.1096 ng/mL, 1089.7160 +/- 736.8395 ng/mL vs 435.9562 +/- 440.8329 ng/mL, P < 0.0001). In ACHBLF patients, plasma sCD163 levels were significantly positively associated with model for end-stage liver disease scores (r = 0.5075, P = 0.008), hepatitis B virus-DNA (r = 0.6827, P < 0.0001), and negatively associated with prothrombin activity (r = -0.3348, P = 0.0347), but had no correlation with total bilirubin (r = 0.2551, P = 0.1122). Furthermore, sCD163 was obviously elevated in non-surviving patients compared with surviving patients with ACHBLF (5344.9080 +/- 1589.5199 ng/mL vs 3641.7333 +/- 1264.5228 ng/mL, P = 0.0321). CONCLUSION: CD163 and sCD163 may be related to disease severity and prognosis in ACHBLF patients. (C) 2013 Baishideng. All rights reserved.
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