期刊
WORLD JOURNAL OF GASTROENTEROLOGY
卷 18, 期 33, 页码 4610-4617出版社
BAISHIDENG PUBL GRP CO LTD
DOI: 10.3748/wjg.v18.i33.4610
关键词
Side population; Cancer stem cells; Self-renewal; Gastric cancer; KATO III
资金
- National Natural Science Foundation of China [81072108, 30600615]
- China Postdoctoral Science Foundation [20090450167, 201003676]
- PhD Programs Fund of the Chinese Ministry of Education [20090201120068]
- International Cooperation Program of Shaanxi Province [2012KW-38]
- Science and Technology Program of Shaanxi Province [2010K14-02]
- Basic Research Funds for the Central Universities
AIM: To investigate whether the side population (SP) cells possess cancer stem cell-like characteristics in vitro and the role of SP cells in tumorigenic process in gastric cancer. METHODS: We analyzed the presence of SP cells in different human gastric carcinoma cell lines, and then isolated and identified the SP cells from the KATO III human gastric cancer cell line by flow cytometry. The clonogenic ability and self-renewal were evaluated by clone and sphere formation assays. The related genes were determined by reverse transcription polymerase chain reaction. To compare tumorigenic ability, SP and non-side population (NSP) cells from the KATO III human gastric cancer cell line were subcutaneously injected into nude mice. RESULTS: SP cells from the total population accounted for 0.57% in KATO III, 1.04% in Hs-746T, and 0.02% in AGS (CRL-1739). SP cells could grow clonally and have self-renewal capability in conditioned media. The expression of ABCG2, MORI, Bmi-1 and Oct-4 was different between SP and NSP cells. However, there was no apparent difference between SP and NSP cells when they were injected into nude mice. CONCLUSION: SP cells have some cancer stem cell-like characteristics ill vitro and can be used for studying the tumorigenic process in gastric cancer. (C) 2012 Baishideng. All rights reserved.
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