期刊
WORLD JOURNAL OF GASTROENTEROLOGY
卷 18, 期 28, 页码 3727-3731出版社
BAISHIDENG PUBL GRP CO LTD
DOI: 10.3748/wjg.v18.i28.3727
关键词
Hepcidin; Expression; In situ hybridization; Immunohistochemistry; Real-time polymerase chain reaction
资金
- Biomarker Society
AIM: To establish methods for quantitative polymerase chain reaction (PCR) for hepcidin using RNAs isolated from paraffin-embedded sections and in situ hybridization of hepatocellular carcinoma (HCC). METHODS: Total RNA from paraffin-embedded sections was isolated from 68 paraffin-embedded samples of HCC. Samples came from 54 male and 14 female patients with a mean age of 66.8 +/- 7.8 years. Quantitative PCR was performed. Immunohistochemistry and in situ hybridization for hepcidin were also performed. RESULTS: Quantitative PCR for hepcidin using RNAs isolated from paraffin-embedded sections of HCC was performed successfully. The expression level of hepcidin mRNA in cancer tissues was significantly higher than that in non-cancer tissues. A method of in situ hybridization for hepcidin was established successfully, and this demonstrated that hepcidin mRNA was expressed in non-cancerous tissue but absent in cancerous tissue. CONCLUSION: We have established novel methods for quantitative PCR for hepcidin using RNAs isolated from paraffin-embedded sections and in situ hybridization of HCC. (C) 2012 Baishideng. All rights reserved.
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