期刊
WORLD JOURNAL OF GASTROENTEROLOGY
卷 16, 期 18, 页码 2283-2290出版社
BAISHIDENG PUBLISHING GROUP INC
DOI: 10.3748/wjg.v16.i18.2283
关键词
Probiotics; Intestinal epithelium; Enteropathogen; Immunomodulation; Adhesion
资金
- National Key Program for Infectious Diseases of China [2008ZX10004-002, 2008ZX10004-009, 2009ZX10004-712]
- Program of Shanghai Subject Chief Scientist [09XD1402700]
AIM: To elucidate the adherence and immunomodulatory properties of a probiotic strain Bifidobacterium lactis (B. lactis) HN019. METHODS: Adhesion assays of B. lactis HN019 and Salmonella typhimurium (S. typhimurium) ATCC 14028 to INT-407 cells were carried out by detecting copies of species-specific genes with real-time polymerase chain reaction. Morphological study was further conducted by transmission electron microscopy. Interleukin-1 beta (IL-1 beta), interleukin-8, and tumor necrosis factor-alpha (TNF-alpha) gene expression were assessed while enzyme linked immunosorbent assay was used to detect IL-8 protein secretion. RESULTS: The attachment of S. typhimurium ATCC 14028 to INT407 intestinal epithelial cells was inhibited significantly by B. lactis HN019. B. lactis HN019 could be internalized into the INT-407 cells and attenuated IL-8 mRNA level at both baseline and S. typhimurium-induced pro-inflammatory responses. IL-8 secretion was reduced while IL-1 beta and TNF-alpha mRNA expression level remained unchanged at baseline after treated with B. lactis HN019. CONCLUSION: B. lactis HN019 does not up-regulate the intestinal epithelium expressed pro-inflammatory cytokine, it showed the potential to protect enterocytes from an acute inflammatory response induced by enteropathogen. (C) 2010 Baishideng. All rights reserved.
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