期刊
WORLD JOURNAL OF GASTROENTEROLOGY
卷 15, 期 23, 页码 2862-2869出版社
BAISHIDENG PUBLISHING GROUP INC
DOI: 10.3748/wjg.15.2862
关键词
Radix Astragali; Traditional Chinese medicine; Obstructive jaundice; Rat; Immune organ; Tumor necrosis factor-alpha; Bax; Nuclear factor-kappa B; Apoptosis; Tissue microarry
资金
- Technological Foundation Project of Traditional Chinese Medicine of Zhejiang Province [2003C130, 2004C142]
- Foundation Project for Medical Science and Technology of Zhejiang Province [2003B134]
- Grave foundation project for Technology and Development of Hangzhou [20031231319]
- Intensive Foundation Project for Technology of Hangzhou [2004Z006]
- Foundation Project for Medical Science and Technology of Hangzhou [2003A004]
- Foundation Project for Technology of Hangzhou [2005224]
AIM: To observe the protective effect of Radix Astragali injection on immune organs (lymph nodes, spleen and thymus) of rats with obstructive jaundice (OJ) and its mechanism. METHODS: SD rats were randomly divided into sham-operation group, model control group and Radix Astragali treatment group. On days 7, 14, 21 and 28 after operation, mortality rate of rats, pathological changes in immune organs, expression levels of Bax and nuclear factor (NF)-kappa B p65 proteins, apoptosis indexes and serum tumor necrosis factor (TNF)-alpha level in spleen and thymus were observed, respectively. RESULTS: Compared to model control group, the number of dead OJ rats in Radix Astragali treatment group decreased (P > 0.05). The TNF-alpha level (27.62 +/- 12.61 v5 29.55 +/- 18.02, 24.61 +/- 9.09 vs 31.52 +/- 10.95) on days 7 and 21, the pathological severity score for spleen [0.0 (0.0) v5 0.0 (2.0) on days 7 and 14 and for lymph nodes [0.0 (1.0) v5 1.0 (2.0), 1.0 (0.0) vs 2.0 (1.0)] on days 21 and 28, the product staining intensity and positive rate of Bax protein in spleen [0.0 (0.0) vs 1.0 (2.0), 0.0 (1.0) vs 2.0 (1.5) and thymus [0.0 (0.0) v5 1.0 (2.0), 0.0 (1.0) v5 2.0 (1.5)] on days 14 and 28, the apoptotic indexes [0.0 (0.0) vs 0.0 (0.01)] in spleen and thymus [0.0 (0.0) vs 0.0 (0.01) on days 14 and 21 were significantly lower in Radix Astragali treatment group than in model control group (P < 0.05). CONCLUSION: Radix Astragali has protective effects on immune organs of OJ rats by relieving the pathological changes in immune organs, reducing TNF-alpha level and inhibiting Bax expression and apoptosis in spleen and thymus. (C) 2009 The WJG Press and Baishideng. All rights reserved.
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