期刊
WATER SCIENCE AND TECHNOLOGY
卷 65, 期 5, 页码 795-801出版社
IWA PUBLISHING
DOI: 10.2166/wst.2012.692
关键词
bacterial consortium; degradation; mixture design; nitrobenzene; Plackett-Burman USA design
资金
- Science and Technology Project of Zhejiang Province [2008C13014-3]
- National Hi-Tech Research and Development Program (863) of China [2007AA06Z329]
- National Natural Science Foundation of China [31070079]
- International Cooperation Project in Science and Technology of Zhejiang Province [2008C14038]
Three bacterial strains, Arthrobacter sp. NB1, Serratia sp. NB2 and Stenotrophomonas sp. NB3, were isolated from contaminated sludge by using nitrobenzene as a sole source of carbon and nitrogen. It was observed that all three strains could degrade nitrobenzene at 400 mg/L initial concentration and mixed-cultivation of these strains could enhance the degradation of nitrobenzene compared with mono-cultivation. Mixture design was used for adjusting the proportions of each strain and the optimal ratio of inoculation size was NB1:NB2:NB3 = 4: 4: 5, where the nitrobenzene degradation percentage was two times higher than for by the single strain. The results of Plackett-Burman design indicated that Mg2+, Ca2+, Fe2+, Zn2+ and Mn2+ had a positive effect on the degradation of nitrobenzene, while Cu2+ and Co2+ had a negative effect on it.
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