期刊
GENOME BIOLOGY
卷 16, 期 -, 页码 -出版社
BIOMED CENTRAL LTD
DOI: 10.1186/s13059-015-0684-3
关键词
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资金
- NIH/NIBIB [K01EB016071]
- NIH [R21EB016980]
- NIBIB
- Common Fund Single Cell Analysis Program
- Columbia University Medical Center
- Howard Hughes Medical Institute
- Cancer Innovative Research Grant [IRG08]
- NIH Pioneer Award [DP1HD084071]
- Packard Fellowship
- Columbia RISE Award
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [1149728] Funding Source: National Science Foundation
Many important biological questions demand single-cell transcriptomics on a large scale. Hence, new tools are urgently needed for efficient, inexpensive manipulation of RNA from individual cells. We report a simple platform for trapping single-cell lysates in sealed, picoliter microwells capable of printing RNA on glass or capturing RNA on beads. We then develop a scalable technology for genome-wide, single-cell RNA-Seq. Our device generates pooled libraries from hundreds of individual cells with consumable costs of $0.10 $0.20 per cell and includes five lanes for simultaneous experiments. We anticipate that this system will serve as a general platform for single-cell imaging and sequencing.
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