期刊
FRONTIERS IN PLANT SCIENCE
卷 6, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fpls.2015.01099
关键词
linkage analysis; molecular marker; QTL; Triticum aestivum; wheat 90K SNP array
资金
- National Natural Science Foundation of China [31461143021]
- Beijing Municipal Science and Technology Project [D151100004415003]
- International Science and Technology Cooperation Program of China [2013DEG30530, 2014DPG31690]
- China Agriculture Research System [CARS-3-1-3]
Identification of genes for yield components, plant height (PH), and yield-related physiological traits and tightly linked molecular markers is of great importance in marker-assisted selection (MAS) in wheat breeding. In the present study, 246 F-8 RILs derived from the cross of Zhou 8425B/Chinese Spring were genotyped using the high-density Illumina iSelect 90K single nucleotide polymorphism (SNP) assay. Field trials were conducted at Zhengzhou and Zhoukou of Henan Province, during the 2012-2013 and 2013-2014 cropping season under irrigated conditions, providing data for four environments. Analysis of variance (ANOVA) of agronomic and physiological traits revealed significant differences (P < 0.01) among RILs, environments, and RILs X environments interactions. Broad-sense heritabilities of all traits including thousand kernel weight (TKW), PH, spike length (SL), kernel number per spike (KNS), spike number/m(2) (SN), normalized difference in vegetation index at anthesis (NDVI-A) and at 10 days post-anthesis (NDVI-10), SPAD value of chlorophyll content at anthesis (Chl-A) and at 10 days post-anthesis (Ch1-10) ranged between 0.65 and 0.94. A linkage map spanning 3609.4 cM was constructed using 5636 polymorphic SNP markers, with an average chromosome length of 171.9 cM and marker density of 0.64 cM/marker. A total of 866 SNP markers were newly mapped to the hexaploid wheat linkage map. Eighty-six OIL for yield components, PH, and yield related physiological traits were detected on 18 chromosomes except 1D, 5D, and 6D, explaining 2.3-33.2% of the phenotypic variance. Ten stable OIL were identified across four environments, viz. QTKW.caas-6A.1, QTKW.caas-7AL, QKNS,caas-4AL, QSN.caas-1 ALI, QPH,caas-4BS.2, QPH.caas-4DS,1, Q,Stcaas-4AS, QSL.caas-4AL.1, QChl-A. caas-5AL, and QCh/-1 0. caas-5BL Meanwhile, 10 OIL-rich regions were found on chromosome 1BS, 2AL (2), 3AL,4AL (2), 4BS, 4DS, 5BL, and 7AL exhibiting pleiotropic effects. These QTL or QTL clusters are tightly linked to SNP markers, with genetic distances to the closest SNPs ranging from 0 to 1.5 cM, and could serve as target regions for fine mapping, candidate gene discovery, and MAS in wheat breeding.
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