期刊
WATER RESEARCH
卷 48, 期 -, 页码 100-107出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.watres.2013.09.021
关键词
Multiresistance; River; ESBLs; qnrVC; Integron; Plasmid typing
资金
- Fundacao para a Ciencia e a Tecnologia (FCT) [SFRH/BD/43468/2008, SFRH/BPD/72256/2010]
- project Sustainable Use of Marine Resources - MARES [CENTRO-07-ST24-FEDER-002033]
- QREN (Mais Centro- Programa Operacional Regional do Centro)
- FEDER
- Observatoire Hommes.Milleux, Estarreja through project ARISKA
In this study we investigated the co-occurrence of resistance to non-beta-lactams among cefotaxime-resistant extended-spectrum beta-lactamase (ESBL) producers (ESBL+) versus non-ESBL producers (ESBI-), from aquatic environments. Higher prevalence of resistance to tetracycline, fluoroquinolones and aminoglycosides were observed in ESBL+. Among ESBL+ resistant to tetracycline (n = 18), tet(A) was detected in 88.9% and tet(B) in 16.7%. Among fluoroquinolone-resistant-ESBL+ (n = 15), aacA4-cr and qnrVC4 were identified in 26.6% and 40% strains, respectively. The qnrVC4 gene was detected for the first time in Pseudomonas sp. and Escherichia coli. Class 1 integrase genes were detected in 56.41% of ESBL+ and in 27.67% ESBL-. Gene cassette arrays identified conferred resistance to aminoglycosides (aadA-type genes and aacA4), trimethoprim (dfrA17), chloramphenicol (catB8), fluoroquinolones (qnrVC4) and beta-lactams (b(laox-10)). Conjugation experiments were performed with CTX-M-producers. Transconjugants showed multiresistance to 3 or more classes of antibiotics, and conjugative plasmids were assigned to IncF, IncK and Inch1 replicons. Results obtained showed that co-selection of resistance to aminoglycosides, quinolones and tetracyclines is prevalent among ESBL-producers and that these features are successfully mobilized by IncF, IncK and Inch 1 conjugative plasmids. This study reinforces the importance of natural aquatic systems as reservoir of mobile genetic platforms carrying multiple resistance determinants. Moreover, to the best of our knowledge, this constitutes the first observation of IncK::CTX-M-3 in Aeromonas hydrophila and the first report of IncK plasmids in Portugal. (C) 2013 Elsevier Ltd. All rights reserved.
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