期刊
WATER RESEARCH
卷 56, 期 -, 页码 304-313出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.watres.2014.03.013
关键词
Multicopper oxidase CueO; Biogenic Mn oxide; gamma-Mn3O4; Mn(II) removal; Mn(II) oxidation
资金
- Chinese National Natural Science Funds [31270105, 40830527]
- National Basic Research Program of China (973 Program) [2010CB126105]
- National High-tech R&D Program of China [2011AA10A205]
Manganese(II) contamination is naturally occurring in many groundwater and surface water sources. Moreover, industrial wastewater is also responsible for much of the Mn(II) contamination. Nowadays, Mn(II) contamination has become a serious environmental problem in some regions of the world. To explore a biological approach for removing excessive amounts of aqueous Mn(II) from water, we found a new biocatalyst multicopper oxidase CueO, which was firstly proved to catalyze the oxidation of Mn(II) both in vitro and in vivo. Subsequently, we established a CueO-mediated catalysis system to prepare biogenic Mn oxide (BioMnO(x)), which was confirmed to be gamma-Mn3O4 by X-ray diffraction. This newly prepared BioMnO(x) consisted of 53.6% Mn(II), 18.4% Mn(III) and 28.0% Mn(IV) characterized by X-ray photoelectron spectroscopy. It exhibited distinct polyhedral structure with nanoparticles of 150-350 nm diameters observed by transmission electron microscopy. Importantly, CueO could remove 35.7% of Mn(II) after a seven-day reaction, and on the other hand, the cueO-overexpressing Escherichia coli strain (ECueO) could also oxidize 58.1% dissolved Mn(II), and simultaneously remove 97.7% Mn(II). Based on these results, we suggest that ECueO strain and CueO enzyme have potential applications on Mn(II) decontamination in water treatment. (c) 2014 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据