期刊
WATER RESEARCH
卷 46, 期 3, 页码 817-827出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.watres.2011.11.056
关键词
LC-MS; Microcystis sp.; Microcystin; Real-time PCR
资金
- Advanced Biomass RD Center, MEST
- DST (Department of Science and Technology), New Delhi [IF 10355]
- UGC (University Grants Commission), New Delhi [P-01/623]
Cyanobacterial blooms in eutrophied water body are generally composed of various genotypes with or without microcystin-producing genes (mcy gene cluster). Thus there is a need for quantification of potent toxin producing strains. The present study aimed at identifying microcystin variants and its producer strains in Durgakund pond, Varanasi, India, based on quantification of cpcBA-IGS and mcyA (condensation domain) genes using real-time PCR and LC-MS. Increase in microcystin concentrations was correlated with increase in mcyA copy number and the level of pigments (chlorophyll a, phycocyanin and carotenoids). Also, selected environmental factors (water temperature, light irradiance, rainfall, pH, N and P) and the concentration of microcystin variants (MC-LR, -RR and -YR) were also assessed in samples during May 2010 to April 2011 to establish the possible correlation among these parameters. Nutrients favored cyanobacterial bloom but it could not be correlated with the levels of microcystin variants and seemed to be geographically specific. Microcystis sp. dominant in the pond comprised potentially toxigenic cells. The ratio of potentially toxigenic Microcystis sp. to that of total Microcystis sp. ranged from 0% to 14%. Such studies paved the way to identify and quantify the most potent microcystin producer in the tropical aquatic body. (C) 2011 Elsevier Ltd. All rights reserved.
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