期刊
WATER RESEARCH
卷 46, 期 16, 页码 5268-5278出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.watres.2012.07.008
关键词
Anaerobic granular sludge; LCFA inhibition; Adsorption isotherms; 16S rDNA profiling; Microscopic techniques
资金
- Spanish Ministry of Science and Innovation [ENE 2007-65850, CTM 2010-18212]
- Department of Universities, Research and Media Society of Catalonia Government [BE-DGR 2008 BE1 00261]
The impact of LCFA adsorption on the methanogenic activity was evaluated in batch assays for two anaerobic granular sludges in the presence and absence of bentonite as synthetic adsorbent. A clear inhibitory effect at an oleate (C18:1) concentration of 0.5 g(C18:1) L-1 was observed for both sludges. Palmitate (C16:0) was confirmed to be the main intermediate of C18:1 degradation in not adapted sludge and its accumulation was further evidenced by fluorescence staining and microscopy techniques. LCFA inhibition could be decreased by the addition of bentonite, reducing the lag-phase and accelerating the kinetics of LCFA degradation, concluding in the importance of the adsorptive nature of the LCFA inhibitory process. Granule morphology and molecular profiling of predominant microorganisms revealed that biomass adaptation to LCFA could modify the intermediates accumulation profiles and process rates. (C) 2012 Elsevier Ltd. All rights reserved.
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