期刊
WATER RESEARCH
卷 44, 期 13, 页码 3915-3923出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.watres.2010.04.015
关键词
Adenosine tri-phosphate (ATP); Bacteria; Drinking water; Flow cytometry; Viability
资金
- TECHNEAU [018320]
There is a widespread need for cultivation-free methods to quantify viability of natural microbial communities in aquatic environments. Adenosine tri-phosphate (ATP) is the energy currency of all living cells, and therefore a useful indicator of viability. A luminescence-based ATP kit/protocol was optimised in order to detect ATP concentrations as low as 0.0001 nM with a standard deviation of <5%. Using this method, more than 100 water samples from a variety of aquatic environments (drinking water, groundwater, bottled water, river water, lake water and wastewater effluent) were analysed for extracellular ATP and microbial ATP in comparison with flow-cytometric (FCM) parameters. Microbial ATP concentrations ranged between 3% and 97% of total ATP concentrations, and correlated well (R(2) = 0.8) with the concentrations of intact microbial cells (after staining with propidium iodide). From this correlation, we calculated an average ATP-per-cell value of 1.75 x 10(-10) nmol/cell. An even better correlation (R(2) = 0.88) was observed between intact biovolume (derived from FCM scatter data) and microbial ATP concentrations, and an average ATP-per-biovolume value of 2.95 x 10(-9) nmol/mu m(3) was calculated. These results support the use of ATP analysis for both routine monitoring and research purposes, and contribute towards a better interpretation of ATP data. (C) 2010 Elsevier Ltd. All rights reserved.
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