期刊
WATER RESEARCH
卷 44, 期 1, 页码 151-158出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.watres.2009.09.013
关键词
Heavy metals; Sulphate reducing bacteria; Fixed bed reactors; Biotreatment; Bioassessment; Caenorhabditis elegans
In this work a batch-optimised mixture (w/w %: 6% leaves, 9% compost, 3% Fe(0), 30% silica sand, 30% perlite, 22% limestone) was investigated in a continuous fixed bed column reactor for the treatment of synthetic acid-mine drainage (AMD). A column reactor was inoculated with sulphate-reducing bacteria and fed with a solution containing sulphate and heavy metals (As(V), Cd, Cr(VI), Cu and Zn). At steady state, sulphate abatement was 50 +/- 10%, while metals were totally removed. A degradation rate constant (k) of 0.015 +/- 0.001 h(-1) for sulphate removal was determined from column data by assuming a first order degradation rate. Reduction of AMD toxicity was assessed by using the nematode Caenorbabditis elegans as a test organism. A lethality assay was performed with the toxicants before and after the treatment, showing that only 5% of the animals were still alive after 48 h in presence of the contaminants, while the percentage increased to 73% when the nematodes were exposed to the solution eluted from the column. (C) 2009 Elsevier Ltd. All rights reserved.
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