Impacts of hydrodynamic shear force on nucleation of flocculent sludge in anaerobic reactor

The Sludge granulation in an anaerobic reactor consists of two steps: nucleation and maturation of nuclei. Nucleation as the starting point is of particular importance. In this paper, the nucleation of flocculent sludge as seed under weak, strong and violent hydrodynamic shear conditions is studied with an original quantitative method, and then the satisfactory linear correlations between the average sludge diameters and the operation time during the nucleation are demonstrated. Nucleation under strong shear conditions with a shear rate of about 8.28 s(-1), corresponding to superficial liquid and gas velocities of 2.66 and 0.24 in h(-1), develops fastest compared to weak shear conditions with a shear rate of about 0.04 s(-1) and violent shear conditions with a shear rate of about 12.42 s(-1) with the average augmentation rate of average sludge diameter of 0.57, 0.40 and 0.41 mu m day(-1) respectively. One of the major mechanisms of the shear force on nucleation is that a high shear force accelerates the extracellular protein secretion of sludge. Although high extracellular protein content benefits nucleation, it is also shown that the extracellular proteins over-produced above around 80.5 mg gVSS(-1) leads nuclei to weaken and inhibit nucleation. So the violent shear force would result in disruption and wash-out of nuclei. However, the high extracellular polymers could intensify the shear force by raising the viscosity in the reactor, thus, in practice, it is important to monitor the shear conditions and extracellular protein content of sludge simultaneously in high rate reactors for stable operation. (C) 2009 Elsevier Ltd. All rights reserved.