期刊
WATER RESEARCH
卷 43, 期 4, 页码 1027-1032出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.watres.2008.12.002
关键词
Silver; ROS (reactive oxygen species); Bactericidal; Superoxide; E. coli; S. aureus
资金
- Seoul R & D Program through Institute of Bioengineering, Seoul National University, Seoul, Korea [10538]
- Pusan National University
- National Research Foundation of Korea [핵06B3406, 과C6A2204] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Silver ions have been widely used as disinfectants that inhibit bacterial growth by inhibiting the essential enzymatic functions of the microorganism via interaction with the thiol-group of L-cysteine. However, silver-ion-mediated perturbation of the bacterial respiratory chain has raised the possibility of reactive oxygen species (ROS) generation. We used bacterial reporter strains specifically responding to superoxide radicals and found that silver-ion-mediated ROS-generation affected bactericidal activity. Almost half the log reduction in Escherichia coli and Staphylococcus aureus populations (model strains for gram negative and positive bacteria, respectively) caused by silver-ion disinfection was attributed to ROS-mediated bactericidal activity. The major form of ROS generated was the superoxide-radical; H2O2 was not induced. Furthermore, silver ions strongly enhanced paraquat-induced oxidative stress, indicating close correlation and synergism between the conventional and ROS-mediated silver toxicity. Our results suggest that further studies in silver-based disinfection systems should consider the oxygen concentration and ROS reaction. (C) 2008 Elsevier Ltd. All rights reserved.
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