期刊
FRONTIERS IN MICROBIOLOGY
卷 5, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fmicb.2014.00774
关键词
filamentous fungi; secondary metabolites; gene clusters; next generation sequencing; genome mining
类别
资金
- NIH Chemistry-Biology Interface Training Grant (NRSA) [GM-08496]
- UCLA Graduate Division
- NIH [1DP1GM106413]
- Australian Research Council (ARC) Discovery Early Career Researcher Award (DECRA)
Genomics has revolutionized the research on fungal secondary metabolite (SM) biosynthesis. To elucidate the molecular and enzymatic mechanisms underlying the biosynthesis of a specific SM compound, the important first step is often to find the genes that responsible for its synthesis. The accessibility to fungal genome sequences allows the bypass of the cumbersome traditional library construction and screening approach. The advance in next-generation sequencing (NGS) technologies have further improved the speed and reduced the cost of microbial genome sequencing in the past few years, which has accelerated the research in this field. Here, we will present an example work flow for identifying the gene cluster encoding the biosynthesis of SMs of interest using an NGS approach. We will also review the different strategies that can be employed to pinpoint the targeted gene clusters rapidly by giving several examples stemming from our work.
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