期刊
VOX SANGUINIS
卷 106, 期 3, 页码 234-241出版社
WILEY
DOI: 10.1111/vox.12092
关键词
platelet concentrates; platelet transfusion; platelet-derived microparticles; soluble CD40L; TRALI
类别
资金
- National Science foundation of China
- Shanghai Municipal Health Bureau
Background and ObjectivesPlatelet-derived microparticles (PMPs) and other proinflammatory mediators, which are accumulated during the storage process, might induce transfusion adverse events. We hypothesized that the PMP primed polymorphonuclear neutrophil (PMN) respiratory burst after the transfusion, which could be linked to the transfusion-related acute lung injury (TRALI). Materials and MethodsThe PMPs were isolated by centrifugation of the platelet-free plasma from 10 apheresis platelet concentrates (A-PLTs) at 20000xg for 1h. The PMPs were counted by flow cytometric analysis, followed by Western blotting, that were performed on isolated PMPs. The soluble CD40 ligand (sCD40L, sCD154) was assayed with ELISA. The priming of the formyl-Met-Leu-Phe (fMLP)-activated PMN respiratory burst was measured with the hydrogen peroxide production. ResultsThe PMP counts increased by 17-folds after 3days of storage. Meanwhile, sCD40L also significantly increased in PMP fraction isolated from the 3-day stored A-PLTs. Furthermore, Western blotting indicated that sCD40L was involved and concentrated in PMP. The PMPs were able to effectively prime the fMLP-activated PMN respiratory burst, which was partly inhibited by CD154 monoclonal antibody or by filtration with 01m membrane. Significant relativity was existed between the PMP counts, sCD40L and priming activity during the A-PLT storage. ConclusionThe platelet-derived microparticles, which carried the sCD40L, accumulated in the apheresis platelet concentrates during the 5days of storage. They primed the fMLP-activated PMN respiration burst, which might be relative to TRALI.
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