期刊
FRONTIERS IN MICROBIOLOGY
卷 6, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fmicb.2015.00746
关键词
nifH; nirK; nirS; nosZ; denitrification; climate change; permafrost; warming
类别
资金
- Department of Energy, Biological Systems Research on the Role of Microbial Communities in Carbon Cycling Program [DE-SC0004601]
- U.S. Department of Energy Office of Science, Office of Biological and Environmental Sciences Division Terrestrial Ecosystem Sciences program [DE-SC0006982]
- National Science Foundation CAREER program [0747195]
- National Parks Inventory and Monitoring Program
- National Science Foundation Bonanza Creek LTER program [1026415]
- National Science Foundation Office of Polar Programs [1203777]
- Division Of Environmental Biology
- Direct For Biological Sciences [0747195, 1026415] Funding Source: National Science Foundation
- Office of Polar Programs (OPP)
- Directorate For Geosciences [1203777] Funding Source: National Science Foundation
Increasing temperatures have been shown to impact soil biogeochemical processes, although the corresponding changes to the underlying microbial functional communities are not well understood. Alterations in the nitrogen (N) cycling functional component are particularly important as N availability can affect microbial decomposition rates of soil organic matter and influence plant productivity. To assess changes in the microbial component responsible for these changes, the composition of the N-fixing (nifH), and denitrifying (nirS, nirK, nosZ) soil microbial communities was assessed by targeted pyrosequencing of functional genes involved in N cycling in two major biomes where the experimental effect of climate warming is under investigation, a tallgrass prairie in Oklahoma (OK) and the active layer above permafrost in Alaska (AK). Raw reads were processed for quality, translated with frameshift correction, and a total of 313,842 amino acid sequences were clustered and linked to a nearest neighbor using reference datasets. The number of OTUs recovered ranged from 231 (NifH) to 862 (NirK). The N functional microbial communities of the prairie, which had experienced a decade of experimental warming were the most affected with changes in the richness and/or overall structure of NifH, NirS, NirK and NosZ. In contrast, the AK permafrost communities, which had experienced only 1 year of warming, showed decreased richness and a structural change only with the nirK-harboring bacterial community. A highly divergent nirK-harboring bacterial community was identified in the permafrost soils, suggesting much novelty, while other N functional communities exhibited similar relatedness to the reference databases, regardless of site. Prairie and permafrost soils also harbored highly divergent communities due mostly to differing major populations.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据