期刊
FRONTIERS IN MICROBIOLOGY
卷 6, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fmicb.2015.00209
关键词
gas chromatography-mass spectrometry; metabolomics; microbial mats; polyhydroxyalkanoates; Roseiflexus; Synechococcus; wax esters
类别
资金
- Genomic Science Program (GSP)
- Office of Biological and Environmental Research (OBER)
- U.S. Department of Energy (DOE)
- Pacific Northwest National Laboratory (PNNL) Foundational Scientific Focus Area
- NSF-DMS [1022836]
- Montana Space Grant Consortium
- Montana Agricultural Experiment Station [911352]
- Division of Chemical Sciences, Geosciences, and Biosciences, Office of Basic Energy Sciences of the DOE [DE-FG02-94ER20137]
- DOE OBER GSP
- OBER
- DOE [DE-AC05-76RLO 1830]
- Division Of Mathematical Sciences
- Direct For Mathematical & Physical Scien [1022836] Funding Source: National Science Foundation
Dynamic environmental factors such as light, nutrients, salt, and temperature continuously affect chlorophototrophic microbial mats, requiring adaptive and acclimative responses to stabilize composition and function. Quantitative metabolomics analysis can provide insights into metabolite dynamics for understanding community response to such changing environmental conditions. In this study, we quantified volatile organic acids, polar metabolites (amino acids, glycolytic and citric acid cycle intermediates, nucleobases, nucleosides, and sugars), wax esters, and polyhydroxyalkanoates, resulting in the identification of 104 metabolites and related molecules in thermal chlorophototrophic microbial mat cores collected over a diel cycle in Mushroom Spring, Yellowstone National Park. A limited number of predominant taxa inhabit this community and their functional potentials have been previously identified through metagenomic and metatranscriptomic analyses and in situ metabolisms, and metabolic interactions among these taxa have been hypothesized. Our metabolomics results confirmed the diel cycling of photorespiration (e.g., glycolate) and fermentation (e.g., acetate, propionate, and lactate) products, the carbon storage polymers polyhydroxyalkanoates, and dissolved gasses (e.g., H-2 and CO2) in the waters overlying the mat, which were hypothesized to occur in major mat chlorophototrophic community members. In addition, we have formulated the following new hypotheses: (1) the morning hours are a time of biosynthesis of amino acids, DNA, and RNA; (2) photo-inhibited cells may also produce lactate via fermentation as an alternate metabolism; (3) glycolate and lactate are exchanged among Synechococcus and Roseiflexus spp.; and (4) fluctuations in many metabolite pools (e.g., wax esters) at different times of day result from species found at different depths within the mat responding to temporal differences in their niches.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据