期刊
VIRUS RESEARCH
卷 189, 期 -, 页码 262-270出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.virusres.2014.05.026
关键词
Coronavirus; Reverse genetics; Infectious clones; Replicons
类别
资金
- Ministry of Science and Innovation of Spain (MCINN) [BIO2010-16705]
- European Community's Seventh Framework Programme (FP7) under the project EMPERIE [HEALTH-F3-2009-223498]
- National Institute of Health (NIH) of USA [2P01AI060699-06A1]
Coronaviruses (CoVs) infect humans and many animal species, and are associated with respiratory, enteric, hepatic, and central nervous system diseases. The large size of the CoV genome and the instability of some Coy replicase gene sequences during its propagation in bacteria, represent serious obstacles for the development of reverse genetic systems similar to those used for smaller positive sense RNA viruses. To overcome these limitations, several alternatives to more conventional plasmid-based approaches have been established in the last 13 years. In this report, we briefly review and discuss the different reverse genetic systems developed for CoVs, paying special attention to the severe acute respiratory syndrome Coy (SARS-CoV). (C) 2014 Elsevier B.V. All rights reserved.
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