期刊
VIRUS RESEARCH
卷 191, 期 -, 页码 21-29出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.virusres.2014.07.022
关键词
Porcine epidemic diarrhea; Coronavirus; Entry; Clathrin; Low pH; Serine protease
类别
资金
- Korea Research Foundation - Korean Government [20120008358, 2011-0023942, 211-2006-2-E00027]
- National Research Foundation of Korea [2011-0023942] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Porcine epidemic diarrhea virus (PEDV), a member of the genus Alphacoronavirus, is a causative agent of porcine enteric disease characterized by acute watery diarrhea and dehydration in sucking piglet. Similar to other coronaviruses, PEDV spike protein mediates its cell entry by binding to cellular receptors and inducing membrane fusion between viral envelopes and cellular membranes. However, the entry mechanism of PEDV is not studied. Here, we determined the entry mechanism of PEDV into Vero cells. Our data confirmed that PEDV entry followed clathrin-mediated endocytosis independence of caveolae-coated pit assembly. The internalized PEDV was co-localized with the clathrin-mediated endocytic marker, but not with the caveolae-mediated endocytic marker. In addition, cells treated with lysosomotropic agents and serine protease inhibitors were resistant to PEDV. Our data revealed that PEDV entry followed clathrin-mediated endocytosis and was dependent on a low pH and serine proteolysis for successful entry into cells. (C) 2014 Elsevier B.V. All rights reserved.
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