期刊
VIRUS RESEARCH
卷 175, 期 1, 页码 71-77出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.virusres.2013.04.009
关键词
Rice stripe virus; Transcription; Termination; Palindromic sequence
类别
资金
- State Basic Research Program of China [2010CB126203, 2012CB722504]
- International Science & Technology Cooperation Program of China [2012DFA30900]
- Special Fund for Agro-scientific Research in the Public Interest of China [201003031]
- National Natural Science Foundation of China [31101414]
- Program for Zhejiang Leading Team of Science and Technology Innovation
- Program for Leading Team of Agricultural Research and Innovation of Ministry of Agriculture, China
Rice stripe virus, the type member of the genus Tenuivirus, has four genomic RNAs. RNAs 2-4 have an ambisense coding strategy and the noncoding intergenic regions (IRs) separating the two ORFs are thought to function in termination of transcription. Sequencing the 3'-untranslated region of transcripts from RNA2 and RNA4 in virus-infected Oryza sativa (the natural host), Nicotiana benthamiana (an experimental host) and Laodelphax striatellus (the vector), showed that the sequences of p2 and pc2 transcripts on RNA2, and p4 and pc4 transcripts on RNA4 terminated with high frequency at a palindromic sequence AUCCGGAU that was located in a region predicted to form a hairpin secondary structure. The AUCCGGAU sequence is highly conserved in RNA2 and RNA4 of different RSV isolates and is also conserved among the corresponding genomic RNAs of other tenuiviruses. p3 transcripts from the three hosts all had the same dominant termination site, while pc3 transcripts from different hosts terminated at different sites. All pc1 3'-UTR sequences ended at the 3'-end of the viral complementary strand of RNA1 (data not shown), indicating that the pc1 transcript may be synthesized by runoff of viral polymerase, but had no characteristic termination sequence. This is the first experimental report determining the exact transcription termination sites of a plant ambisense virus, and has implications for understanding the transcription of RSV as well as other plant viruses with an ambisense coding strategy. (C) 2013 Elsevier B.V. All rights reserved.
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