期刊
VIRUS RESEARCH
卷 176, 期 1-2, 页码 216-222出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.virusres.2013.06.010
关键词
Duck Tembusu virus; Replication; Pathogenicity; Antibody-dependent enhancement; Mouse model
类别
资金
- Key S&T Project of Gansu Province for Talents, China [1013JHTA008]
- Basic Scientific Research Fund of the Chinese Academy of Agricultural Sciences [0032011027]
- National Natural Science Foundation of China [31101839]
Duck Tembusu virus (DTMUV) is a newly emerging infectious agent in China. Since 2010, this presumably arthropod-borne virus has caused severe disease in duck flocks. A cell-adapted DTMUV, designated AH10, has been developed. For this particular virus, replication, pathogenicity, and antibody-dependent enhancement (ADE) infection mediated by a specific antibody was investigated in mammalian cells and mice. The virus propagated in Vero or Hepa1-6 cells with a stable titer, and induced a visible cytopathic effect. Expression of pro- and anti-inflammatory cytokine genes in Hepa1-6 cells was elevated during early infection stages. AH10 was able to replicate in the brain, spleen, liver and kidneys of mice, and resulted in a systemic infection by day 5 when administered intracerebrally. When, naive mice were first transfused intraperitoneally with virus-specific antisera, upon subsequent infection, ADE of virus symptoms was observed. Mice displayed clinical symptoms by day 3 post-infection; weight loss in the AH10-specific antibody-treated group was observed 2-3 days earlier than that in the control groups. These findings indicate that AH10 was able to replicate in the mouse model, and that anti-AH10 antibodies caused ADE. The molecular basis of pathogenicity and ADE in the mouse model should be investigated in the future. (C) 2013 Elsevier B.V. All rights reserved.
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