期刊
VIRUS RESEARCH
卷 167, 期 2, 页码 173-178出版社
ELSEVIER
DOI: 10.1016/j.virusres.2012.04.012
关键词
Chronic bee paralysis virus (CBPV); Naked RNAs; In vivo experimental model; Negative-strand RNA; Replication, Viral particle
类别
资金
- ANSES
Chronic paralysis is an infectious and contagious disease of the honeybee (Apis mellifera L.) and is caused by the chronic bee paralysis virus (CBPV). This disease leads to death in adult bees and is therefore a serious threat for colony health. CBPV is a positive single-stranded RNA virus and its genome is composed of two RNA segments, RNA 1 and RNA 2,3674 nt and 2305 nt, respectively. Although CBPV shares some characteristics with viruses classified into families Nodaviridae and Tombusviridae, it has not been assigned to any viral taxa yet. The characterisation of CBPV proteins and their functions are needed to better understand the mechanisms of CBPV infection. However, since honeybee cell lines are not yet available, experimental infection of adult bees is the only method currently available to propagate the virus. With the objective of studying CBPV proteins using the viral genome, we used experimental infection in adult bees to evaluate the infectivity of naked CBPV RNAs by direct inoculation. Our results demonstrated that an injection of naked RNAs, ranging from 10(9) to 10(10) CBPV copies, caused chronic paralysis. Bees inoculated with naked RNA showed chronic paralysis signs 5 days after inoculation. Moreover, injected RNAs replicated and generated viral particles. We therefore provide an in vivo experimental model that will be useful tool for further studies by using a reverse genetics system. (c) 2012 Elsevier B.V. All rights reserved.
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