期刊
VIRUS RESEARCH
卷 156, 期 1-2, 页码 141-146出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.virusres.2010.12.011
关键词
Hepatitis E virus (HEV); Chimeric viruses; Cross-species infection; Pigs; Host range
类别
资金
- National Institutes of Health [R01AI050611, R01AI074667]
Genotypes 1 and 2 hepatitis E virus (HEV) infect only humans whereas genotypes 3 and 4 HEV infect both humans and pigs. To evaluate the mechanism of cross-species HEV infection between humans and swine, in this study we constructed five intergenotypic chimeric viruses and tested for their infectivity in vitro and in pigs. We demonstrated that chimeric viruses containing the ORF2 capsid gene either alone or in combination with its adjacent 5' junction region OR) and 3' noncoding region (NCR) from a genotype 4 human HEV in the backbone of a genotype 3 swine HEV are replication-competent in Huh7 cells and infectious in HepG2/C3A cells and in pigs, and thus supporting the hypothesis that genotypes 3 and 4 human HEV are of swine origin. However, chimeric viruses containing the JR+ ORF2 + 3' NCR of genotypes :3 or 4 HEV in the backbone of genotype 1 human HEV failed to infect pigs, suggesting that other genomic regions such as 5' NCR and ORF1 may also be involved in HEV cross-species infection. The results from :his study provide the first experimental evidence of the exchangeability of the capsid gene between genotype 3 swine HEV and genotype 4 human HEV, and have important implications for understanding the mechanism of HEV cross-species infection. (C) 2010 Elsevier B.V. All rights reserved.
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