期刊
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
卷 5, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fcimb.2015.00059
关键词
Vibrio alginolyticus; adhesion; flagellar assembly pathway; environmental stresses
资金
- National Natural Science Foundation of China [31272699, 31272669, 31202030]
- Science and Technology Program of Xiamen [3502Z20133016]
- Science and Technology Program of the Ocean and Fisheries Bureau of Xiamen [201357]
- Science and Technology Program of Xiamen Southern Oceanographic Center [14CZP032HJ06, 14PYY050SF03]
- Key Laboratory of Healthy Mariculture for the East China Sea [2013ESHML02]
Adhesion is an important virulence factor of Vibrio alginolyticus. This factor may be affected by environmental conditions; however, its molecular mechanism remains unclear. In our previous research, adhesion deficient strains were obtained by culturing V. alginolyticus under stresses including Cu, Pb, Hg, and low pH. With RNA-seq and bioinformatics analysis, we found that all of these stress treatments significantly affected the flagellar assembly pathway, which may play an important role in V. alginolyticus adhesion. Therefore, we hypothesized that the environmental stresses of the flagellar assembly pathway may be one way in which environmental conditions affect adhesion. To verify our hypothesis, a bioinformatics analysis, QPCR, RNAi, in vitro adhesion assay and motility assay were performed. Our results indicated that (1) the flagellar assembly pathway was sensitive to environmental stresses, (2) the flagellar assembly pathway played an important role in V. alginolyticus adhesion, and (3) motility is not the only way in which the flagellar assembly pathway affects adhesion.
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