期刊
VIRUS RESEARCH
卷 150, 期 1-2, 页码 129-134出版社
ELSEVIER
DOI: 10.1016/j.virusres.2010.03.005
关键词
Hepatitis B virus; Hepatitis B core protein; Nucleocapsid; Antiviral peptide
类别
资金
- National Natural Science Foundation of China [30872233, 30700697]
The nucleocapsids formation is a pivotal step of hepatitis B virus (HBV) life cycle. The inhibition of HBV nucleocapsids assembly is a promising strategy for the anti-HBV treatment. HBc78-117 is an internal fragment of hepatitis B core protein (HBc). In this study, we used lentiviral vector to deliver HBc78-117 cDNA sequence into HepG2.2.15 cells and examined the effect of H8c78-117 on HBV replication. We confirmed by immunoprecipitation analysis that HBc78-117 interacted with full-length HBc in HepG2.2.15 cells. The nucleocapsids and HBV DNA replication intermediates were markedly reduced in the cells expressing HBc78-117, although HBV pregenome RNA was not affected. The level of HBV DNA was also significantly reduced in culture supernatant. These suggest that HBc78-117 can inhibit HBV DNA replication by interfering with nucleocapsids assembly. (C) 2010 Elsevier B.V. All rights reserved.
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