期刊
VIRUS RESEARCH
卷 144, 期 1-2, 页码 96-106出版社
ELSEVIER
DOI: 10.1016/j.virusres.2009.04.004
关键词
2-DE; FSI-MS/MS; Fusarium graminearum virus-DK21; Hypovirulence
类别
资金
- Agriculture Specific Research
- Rural Development Administration
- Korea Research Foundation grant (MOEHRD, Basic Research Promotion Fund [KRF-2005-217-F00001]
- Korea Science and Engineering Foundation (KOSEF) [RII-2008-062-02003-0]
- Korean Government (MEST).
- SYC
- KML
- JSY
- MIS
- Ministry of Education through Brain Korea 21 Project
Fusarium graminearum virus-DK21 (FgV-DK21), which infects the plant pathogenic E graminearum, perturbs host developmental processes such as sporulation, morphology, pigmentation, and attenuates the virulence (hypovirulence) of the host. To identify the differentially expressed F graminearum proteins by FgV-DK21 infection, we have used two-dimensional electrophoresis with mass spectrometry using proteins extracted from virus-free and FgV-DK21-infected strains. A total of 148 spots showing an altered expression were identified by PDQuest (TM) program. Among these spots, 33 spots were exclusively analyzed including 14 spots from FgV-DK21-infected and 19 spots from virus-free strains by ESI-MS/MS analyses and successfully identified 23 proteins. Seven proteins including sporulation-specific gene SPS2, triose phosphate isomerase, nucleoside diphosphate kinase, and woronin body major protein precursor were induced or significantly up-regulated by FgV-DK21 infection. A significant decrease or down regulation of 16 proteins including enolase, saccharopine dehydrogenase, flavohemoglobin, mannitol dehydrogenase and malate dehydrogenase caused by FgV-DK21 infection was also identified. Variations of protein expression were also further investigated at the mRNA level by real-time RT-PCR analysis, which confirmed the proteomic data for 9 out of the representative 11 selected proteins including 5 proteins from up-regulated group and 6 proteins from down-regulated group. Further investigation of these differentially expressed proteins will provide novel insights into the molecular responses of E graminearum to FgV-DK21 infection. (C) 2009 Elsevier B.V. All rights reserved.
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