期刊
VIRUS RESEARCH
卷 143, 期 1, 页码 125-129出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.virusres.2009.03.002
关键词
RNA virus; Norovirus; pH-independent entry; Macrophages; Dendritic cells; Enteric virus
类别
资金
- NIH/NIAID [1-U54-AI-057153]
- University of Michigan Human Genetics [NIH T32 GM 07544]
Murine norovirus (MNV) is a recently discovered mouse pathogen. Unlike the fastidious human noroviruses that cause the overwhelming majority of non-bacterial gastroenteritis worldwide, MNV readily infects cells in culture. Its replication in primary murine macrophages and dendritic cells and their derived cell lines allows the study of norovirus cell entry for the first time. in this study we determined the role of pH during MNV-1 infection since the low pH environment of endosomes often triggers uncoating of viruses. We demonstrated that MNV-1 viral titers by plaque assay and expression of the non-structural protein VPg by immunofluorescence were not affected by pH in cultured and primary macrophages and dendritic cells in the presence of two known endosome acidification inhibitors, bafilomycin A1 and chloroquine. These data indicate that MNV-1 enters permissive cells in a pH-independent manner. (C) 2009 Elsevier B.V. All rights reserved.
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