期刊
VIRUS RESEARCH
卷 142, 期 1-2, 页码 10-18出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.virusres.2008.12.016
关键词
APMV; Avian paramyxovirus; Paramyxoviridae; Paramyxovirinae; Genome sequence
类别
资金
- MAID [N01A060009]
- NIH
The complete genome consensus sequence was determined for avian paramyxovirus (APMV) serotype 9 prototype strain PMV-9/domestic Duck/New York/22/78. The genome is 15,438 nucleotides (nt) long and encodes six non-overlapping genes in the order of 3'-N-P/V/W-M-F-HN-L-5' with intergenic regions of 0-30 nt. The genome length follows the rule of six and contains a 55-nt leader sequence at the 3' end and a 47-nt trailer sequence at the 5' end. The cleavage site of the F protein is I-R-E-G-R-I down arrow F, which does not conform to the conventional cleavage site of the ubiquitous cellular protease furin. The virus required exogenous protease for in vitro replication and grew only in a few established cell lines, indicating a restricted host range. Alignment and phylogenetic analysis of the predicted amino acid sequences of APMV-9 proteins with the cognate proteins of viruses of all five genera of family Paramyxoviridae showed that APMV-9 is more closely related to APMV-1 than to other APMVs. The mean death time in embryonated chicken eggs was found to be more than 120 h, indicating APMV-9 to be avirulent for chickens. (C) 2009 Elsevier B.V. All rights reserved.
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