期刊
VIRUS RESEARCH
卷 146, 期 1-2, 页码 73-80出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.virusres.2009.09.002
关键词
Cocksfoot mottle virus; Sobemovirus; Ribosomal frameshift; RNA structure; RNA stem-loop; Local and systemic infection
类别
资金
- Estonian Science Foundation [7363]
The -1 programmed ribosomal frameshifting (-1 PRF) mechanism utilized by many viruses is dependent on a heptanucleotide slippery sequence and a downstream secondary structure element. In the current study, the RNA structure downstream from the slippery site of cocksfoot mottle sobemovirus (CfMV) was proven to be a 12 bp stem-loop with a single bulge and a tetranucleotide loop. Several deletion and insertion mutants with altered stem-loop structures were tested in wheat germ extract (WGE) for frameshifting efficiency. The impact of the same mutations on virus infectivity was tested in oat plants. Mutations shortening or destabilizing the stem region reduced significantly but did not abolish -1 PRF in WGE. The same mutations proved to be deleterious for virus infection. However, extending the loop region to seven nucleotides had no significant effect on frameshifting efficiency in WGE and did not hamper virus replication in infected leaves. This is the first report about the experimentally proven RNA secondary structure directing -1 PRF of sobemoviruses. (C) 2009 Elsevier B.V. All rights reserved.
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