期刊
VIRUS GENES
卷 37, 期 3, 页码 380-391出版社
SPRINGER
DOI: 10.1007/s11262-008-0278-1
关键词
PRRSV; structural protein genes; virus neutralization; gene-swapping
资金
- United States Department of Agriculture Formula
- Iowa Livestock Health Advisory Council
- Iowa Pork Producers Association
To assess the role of each envelope-associated protein (i.e., ORFs 2-6 products) of type 2 PRRSV in cross neutralization mediated by antibody, chimeric mutants were generated by replacing ORFs of a VR2332-based infectious clone with those of JA142, SDSU73, PRRS124, or 2M11715 that are genetically and antigenically distinct from VR2332 and two-way neutralization assays were performed on those mutants using VR2332, JA142, SDSU73, or PRRS124 antisera. All ORF 5-replaced mutants showed increased susceptibility or resistance against homologous or heterologous antisera, respectively, in comparison to that of the donor strains, but failed to achieve a complete reversion of cross neutralization. In contrast, substitution of ORFs 3-6 completely reversed the susceptibility of the virus to neutralization by antibody. Changes in ORFs 3, 5, and 6 were additively responsible for reversion of the susceptibility, suggesting that the genetic similarity of these ORFs should be considered for better cross neutralization between two different type 2 PRRS viruses.
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