期刊
VIROLOGY
卷 449, 期 -, 页码 109-119出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2013.11.006
关键词
M-PMV; Envelope; Gag; Anterograde transport; Cytoskeleton; Live cell-imaging
类别
资金
- NIH [CA-27834]
- National Cancer Institute and Czech Ministry of Education [LN12011]
- Czech Science Foundation [P302/12/1895]
- American Society for Microbiology Robert D. Watkins Fellowship
The intracellular transport of Mason-Pfizer monkey virus (M-PMV) assembled capsids from the pericentriolar region to the plasma membrane (PM) requires trafficking of envelope glycoprotein (Env) to the assembly site via the recycling endosome. However, it is unclear if Env-containing vesicles play a direct role in trafficking capsids to the PM. Using live cell microscopy, we demonstrate, for the first time, anterograde co-transport of Gag and Env. Nocodazole disruption of microtubules had differential effects on Gag and Env trafficking, with pulse-chase assays showing a delayed release of Env-deficient virions. Particle tracking demonstrated an initial loss of linear movement of GFP-tagged capsids and mCherry-tagged Env, followed by renewed movement of Gag but not Env at 4 h post-treatment. Thus, while delayed capsid trafficking can occur in the absence of microtubules, efficient anterograde transport of capsids appears to be mediated by microtubule-associated Env-containing vesicles. (C) 2013 Elsevier Inc. All rights reserved.
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